Overcoming factors limiting high-solids fermentation of lignocellulosic biomass to ethanol

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Overcoming factors limiting high-solids fermentation of lignocellulosic biomass to ethanol.

Simultaneous saccharification and fermentation (SSF) of solid biomass can reduce the complexity and improve the economics of lignocellulosic ethanol production by consolidating process steps and reducing end-product inhibition of enzymes compared with separate hydrolysis and fermentation (SHF). However, a long-standing limitation of SSF has been too low ethanol yields at the high-solids loading...

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Ultrasonically Assisted Conversion of Lignocellulosic Biomass to Ethanol

In order to improve the bioconversion of lignocellulosic materials in an environmentally friendly manner, we choose to use the ultrasonic energy. Since enzymatic hydrolysis of cellulose represents a significant part of production costs, it is economically advantageous to develop a technique for reducing the cellulolitic enzyme loading. Thus, the sonication was employed in a two steps concept pr...

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A modified method for calculating practical ethanol yield at high lignocellulosic solids content and high ethanol titer.

A modified method for calculating practical ethanol yield in the simultaneous saccharification and fermentation (SSF) at high lignocellulosic solids content and high ethanol titer is proposed considering the liquid volume change caused by high titer ethanol generation and the water consumed during cellulose degradation. This modified method was applied to determine the practical ethanol yields ...

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Laboratory-scale method for enzymatic saccharification of lignocellulosic biomass at high-solids loadings

BACKGROUND Screening new lignocellulosic biomass pretreatments and advanced enzyme systems at process relevant conditions is a key factor in the development of economically viable lignocellulosic ethanol. Shake flasks, the reaction vessel commonly used for screening enzymatic saccharifications of cellulosic biomass, do not provide adequate mixing at high-solids concentrations when shaking is no...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2017

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.1704652114